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H Kaur Book Spectroscopy Pdf Download

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raman spectroscopy can also distinguish between different excipients ( lim, 2012 ). if the excipients were mixed together before production, it would be nearly impossible to identify them using other techniques.


colorimetry is a common and inexpensive technique used in drug analysis. the use of a dye in the test solution can be used to identify a drug. colorimetry is most commonly used to screen for the presence of impurities, but it can be used to test the purity of a drug or to measure its concentration ( kaur et al., 2010 ). the most commonly used dye is methylene blue, although others are available ( usp, 2007 ).


concentration methods are often used in conjunction with colorimetry to quantify a drug in a sample. concentration methods include enzyme-linked immunosorbent assays, high-performance liquid chromatography, and reversed-phase liquid chromatography ( kaur et al., 2010 ). enzyme-linked immunosorbent assays, also known as immunoassays, are used to detect the presence of an active ingredient or an impurity in a sample. high-performance liquid chromatography (hplc) uses an automated and faster technique to separate compounds by separating them based on their molecular weight. in reversed-phase liquid chromatography (rplc), the separation is achieved by using an organic solvent, such as acetonitrile, in conjunction with a polar solvent, such as water, to extract the active ingredient ( kaur et al.


spectroscopic techniques, such as uv-visible spectroscopy, infrared spectroscopy, and raman spectroscopy, can be used to detect active ingredients and measure the concentration of a drug. uv-visible spectroscopy is used to detect the presence of specific compounds and can measure the purity of a drug ( usp, 2007 ). infrared spectroscopy can also be used to detect specific compounds ( usp, 2007 ). raman spectroscopy can also be used to identify a compound and measure its concentration ( usp, 2007 ). 84d34552a1