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12. what is meant by the following curve? a) resultant amplitude curveb) resultant interference patternc) resultant intensity curved) resultant diffraction patternanswer: aclarification: the following graph shows the resultant intensity variation of the pattern observed by a plane transmission grating. as we can see, there are three maxima and two minima.
13. what would the resulting curve look like if the grating was made of glass? a) resultant amplitude curveb) resultant interference patternc) resultant intensity curved) resultant diffraction patternanswer: dclarification: the following graph shows the resultant intensity variation of the pattern observed by a plane transmission grating. as we can see, there are three maxima and two minima.
14. what is meant by a ‘hologram’? a) resultant amplitude curveb) resultant interference patternc) resultant intensity curved) resultant diffraction patternanswer: dclarification: in this case we have a holographic image of a yeast cell. the image shows the surface contour of the cell. the image was obtained by a metal-dielectric grating illuminated by a laser beam with a gaussian profile. the gaussian beam was generated by the diffraction of a second (spatial) laser beam by the grating.
15. which of the following statements about the technique presented is not correct? a) it is dependent on the existence of sufficient fluorescent probes within the sampleb) it uses a holographic plate, or hologramc) the image can be reconstructed using a digital camerad) it relies on the nonlinearity of the microscope optics to create the super-resolution imageanswer: bclarification: the hologram is just another name for the grating. the grating is a periodic modulation of the refractive index of a material. the grating acts as a lens and allows for the magnification of the pattern of fluorescence intensity that is generated by the illumination by a laser beam. the magnified pattern is a single fourier component of the hologram, and the magnified pattern can be reconstructed using a digital camera. 84d34552a1
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